dose-response function Search Results


dose–response curvesfinhibition function  (GraphPad Software Inc)
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GraphPad Software Inc dose–response curvesfinhibition function
Dose–Response Curvesfinhibition Function, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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non-linear curve fitting/growth/sigmoidal/dose–response fitting functions originpro 8.6.0 b70  (OriginLab corp)
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OriginLab corp non-linear curve fitting/growth/sigmoidal/dose–response fitting functions originpro 8.6.0 b70
Non Linear Curve Fitting/Growth/Sigmoidal/Dose–Response Fitting Functions Originpro 8.6.0 B70, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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4 parameter, nonlinear regression of dose response inhibition  (GraphPad Software Inc)
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GraphPad Software Inc 4 parameter, nonlinear regression of dose response inhibition
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
4 Parameter, Nonlinear Regression Of Dose Response Inhibition, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nonlinear regression to a four parameter, variable slope, doseresponse-inhibition function in  (GraphPad Software Inc)
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GraphPad Software Inc nonlinear regression to a four parameter, variable slope, doseresponse-inhibition function in
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
Nonlinear Regression To A Four Parameter, Variable Slope, Doseresponse Inhibition Function In, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nonlinear regression function dose–response – inhibition; inhibitor vs. normalized response  (GraphPad Software Inc)
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GraphPad Software Inc nonlinear regression function dose–response – inhibition; inhibitor vs. normalized response
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
Nonlinear Regression Function Dose–Response – Inhibition; Inhibitor Vs. Normalized Response, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dose–response function  (Hirasawa Works)
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Hirasawa Works dose–response function
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
Dose–Response Function, supplied by Hirasawa Works, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quadratic dose-response form  (National Research Council Canada)
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National Research Council Canada quadratic dose-response form
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
Quadratic Dose Response Form, supplied by National Research Council Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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logistic dose–response function equation 8013  (SYSTAT)
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SYSTAT logistic dose–response function equation 8013
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
Logistic Dose–Response Function Equation 8013, supplied by SYSTAT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dose–response curve fit with a 5-parameter non-linear function using graphpad prism 8  (GraphPad Software Inc)
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GraphPad Software Inc dose–response curve fit with a 5-parameter non-linear function using graphpad prism 8
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
Dose–Response Curve Fit With A 5 Parameter Non Linear Function Using Graphpad Prism 8, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dose response function fitting in originpro 9.7  (OriginLab corp)
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OriginLab corp dose response function fitting in originpro 9.7
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
Dose Response Function Fitting In Originpro 9.7, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xy analysis for nonlinear regression curve and the 3-parameters dose-response stimulation function  (GraphPad Software Inc)
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GraphPad Software Inc xy analysis for nonlinear regression curve and the 3-parameters dose-response stimulation function
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
Xy Analysis For Nonlinear Regression Curve And The 3 Parameters Dose Response Stimulation Function, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dose-response function  (GraphPad Software Inc)
90
GraphPad Software Inc dose-response function
(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus <t>inhibition</t> assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.
Dose Response Function, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus inhibition assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.

Journal: PLoS ONE

Article Title: A Novel Compound from the Mushroom Cryptoporus volvatus Inhibits Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) In Vitro

doi: 10.1371/journal.pone.0079333

Figure Lengend Snippet: (A and B) The C M-H-L-5 blocked PRRSV Ch1a replication in Marc-145 cells. Marc-145 cells were infected with PRRSV Ch1a at an MOI of 0.1, and then treated with IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. At 24 h p.i., cells were fixed and analyzed by IFA using antibody against PRRSV N protein (A), and virus yield in the supernatants was also quantified (B). Culture treated with normal saline was set up as negative control (0 mg/ml), and treated with INF-α was set up as positive control. The brightness of the fluorescence represents the level of the virus. Results are from three independent experiments, each of which was conducted in triplicate. (C) The C M-H-L-5 potently inhibited both PRRSV HV and VR2332 replication in PAMs. A similar virus inhibition assay was performed with PAM cells infected with PRRSV strain HV or VR2332 at an MOI of 0.1 in the presence of IFN-a (10 units/µl) or the C M-H-L-5 at various concentrations. (D) Determination of cytotoxicity of the C M-H-L-5 by MTT assay. PAMs or Marc145 cells were incubated with various concentrations of the C M-H-L-5 or the control normal saline for 48 h prior to the MTT assay. Data are representative of three independent experiments (mean ± SD). Statistical significance was analyzed by Student’s t test. *P<0.05; **P<0.01; ***P<0.001.

Article Snippet: The 50% effective concentration (EC 50 ) was determined using a 4 parameter, nonlinear regression of dose response inhibition by plotting log (inhibitor(-concentration)) vs. virus titer (variable slope) using GraphPad Prism (GraphPad Software, San Diego, CA).

Techniques: Infection, Virus, Saline, Negative Control, Positive Control, Fluorescence, Inhibition, MTT Assay, Incubation, Control